What Type Of Instruments Are Used With Real-Time PCR?

• Real-time PCR instruments consist of THREE main components:

1. Thermal Cycler (PCR machine)
2. Optical Module (to detect fluorescence in the tubes during the run)
3. Computer (to translate the fluorescence data into meaningful results)

• The real-time software converts the fluorescent signals in each well to meaningful data.

1. Set up PCR protocol
2. Set up plate layout
3. Collect data
4. Analyze data
5. Interpret