The steps are:
- Open the sterilised bottle as described above.
- Fill the bottle by holding it by the lower part and submerging it to a depth of about 20 cm, with the mouth facing slightly upwards. If there is a current, the bottle mouth should face towards the current (Figure 17.5).
- The bottle should then be capped or stoppered.

Figure 17.5 Sampling of water from surface water (rivers, ponds etc.). (Source: as Figure 17.4)